Messi, arguably the best player in the world, and Bale, the most expensive player ever, were joined by Manchester United striker Javier Hernandez, former Everton midfielder Tim Cahill and AC Milan hotshot Stephan El Shaarawy for the advert.
Canadian rapper Drake, a huge football fan who helped introduce the game at video game conference E3, also features on the video.
After FIFA revealed their player rankings for the new game, it was somewhat surprising to see the £86million Bale way down in 17th place on the list, below the likes of Messi, Cristiano Ronaldo and Robin van Persie.
He is ranked at 87 (out of 99), level with the man who has just exited Real Madrid for Arsenal, Mesut Ozil.
FIFA 14 will be released in the UK on September 27.
In the desert: Lionel Messi opens the advert driving a van
Most expensive: FIFA 14 cover star Gareth Bale is also in the advert
Couch potatoes: Javier Hernandez and Tom Cahill were part of the video
Surprise performance: Canadian rapper Drake is a big football fan
