The group from Florida, known as ROAR, were pictured in the capital to shoot the calendar which is scheduled to be launched in August.
The photo shoot comes after the Jacksonville Jaguars agreed to play one of their eight NFL home matches at Wembley Stadium for each of the next four years.
On tour: The Jacksonville Jaguars cheerleaders shot their new calendar in London
Drawing a crowd: The cheerleaders were at Piccadilly Circus on Sunday
Taxi: The calendar will take in some of London's famous sights and landmarks
Shoot: The Jacksonville Jaguars cheerleaders are called the ROAR
Worth the wait: The calendar will be launched in August just before the start of the NFL season in September
The new NFL season will kick off on September 5 when reigning NFL champions Baltimore Ravens travel to the Denver Broncos.
Phone booth: The cheerleaders have been shooting their new calendar over Bank Holiday weekend in London
Cruise: The ROAR shot some of their calendar from a boat along the River Thames on Monday
London calling: Jacksonville will play one home match at Wembley for each of the next four seasons
Routine: The ROAR in action back home in Florida
International Series: Wembley has hosted regular season NFL matches since 2007